Coding

Part:BBa_K3868048:Design

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-15)


paraB-λ-red


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 181
    Illegal EcoRI site found at 1690
    Illegal PstI site found at 1185
    Illegal PstI site found at 1432
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 181
    Illegal EcoRI site found at 1690
    Illegal PstI site found at 1185
    Illegal PstI site found at 1432
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 181
    Illegal EcoRI site found at 1690
    Illegal BamHI site found at 120
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 181
    Illegal EcoRI site found at 1690
    Illegal PstI site found at 1185
    Illegal PstI site found at 1432
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 181
    Illegal EcoRI site found at 1690
    Illegal PstI site found at 1185
    Illegal PstI site found at 1432
    Illegal AgeI site found at 1612
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Recombinant enzyme, gene from plasmid pKD46

References